Beitrag in Konferenzband
Deep sequencing of the shoot apical meristem transcriptome of columnar apple trees (Malus x domestica)


Details zur Publikation
Autorenliste: Krost C., Petersen R., Schmidt E.R., Braun P.
Jahr der Veröffentlichung: 2014
Buchtitel: Proceedings of the IInd International Symposium on Biotechnology of Fruit Species : Nelson, New Zealand, March 25-29, 2012
Serientitel: Acta Horticulturae
Serienzählung: 1048
Erste Seite: 103
Letzte Seite: 106
Verlag: ISHS
Ort: Leuven
ISBN: 978-94-6261-036-1
DOI: 10.17660/ActaHortic.2014.1048.11
Sprachen: Englisch

Abstract

Columnar apple trees (Malus × domestica) are an interesting
research object for plant breeders as well as molecular geneticists
because of their striking growth habit and the fact that it is caused by
the presence of a single dominant allele of the Co gene.
The phenotype is characterized by nearly absent lateral branching and an
unusual thick stem.
Since almost nothing is known about the molecular function(s) of this
gene, we performed Illumina RNA-seq next generation sequencing to
compare the transcriptomes of columnar and non-columnar shoot apical
meristems (SAMs). With this approach, we found hundreds of genes to be
differentially regulated, even at different sample collection dates.
Functional assignment using gene ontology (GO) tools revealed that many
of these genes can potentially be associated with the columnar
phenotype.
There is noticeable evidence that the expression levels of many genes
involved in phytohormone synthesis and signaling are changed.
Thus, we hypothesize that columnar growth is in part a consequence of a
disregulated phytohormone network.