Zeitschriftenaufsatz

Temperature-dependent production of pseudoinfectious dengue reporter virus particles by complementation


Details zur Publikation

AutorenlisteAnsarah-Sobrinho C., Nelson S., Jost C.A., Whitehead S.S., Pierson T.C.

Jahr der Veröffentlichung2008

QuelleVirology

Bandnummer381

Erste Seite67

Letzte Seite74

ISSN0042-6822

DOI10.1016/j.virol.2008.08.021

SprachenEnglisch


Abstract
Dengue Virus (DENV) is a mosquito-borne flavivirus responsible for 50 to 100 million human infections each year, highlighting the need for a safe and effective vaccine. In this study, we describe the production of pseudoinfectious DENV reporter virus particles (RVPs) using two different genetic complementation approaches, including the creation of cell lines that release reporter viruses in an inducible fashion. In Contrast to Studies with West Nile virus (WNV), production of infectious DENV RVPs was temperature-dependent; the yield Of infectious DENV RVPs at 37 degrees C is significantly reduced in comparison to experiments conducted at lower temperatures Or With WNV. This reflects both a significant reduction in the late Of infectious DENV RVP release over time, and the more rapid decay of infectious DENV RVPs at 37 degrees C. Optimized production approaches allow the production of DENV RVPs with titers Suitable for the study Of DENV entry, assembly, and the analysis of the humoral immune response of infected and vaccinated individuals. (C) 2008 Published by Elsevier Inc